LLG » Cooperations » Multidimensional proteome analysis system

Multidimensional proteome analysis system: Innovative coupling of novel electrophoretic enhancement/separation, Surface Enhanced Raman Spectroscopy and liquid beam desorption mass spectrometry The project is supported by the German Federal Land Lower Saxony within the bounds of the biophotonic-initiative.
In the past years, the proteome research became more and more important all over the world because of its major importance for pharmaceutical research to identify drug targets, as well as because of its relevance for early diagnoses of illnesses by means of specific detections of biomarkers. But in the moment, none of the used proteome analysis technologies possesses the necessary high throughput, the selectivity and sensitivity and the essential level of automation required to select, identify and quantify hundreds and thousands of different proteins in an acceptable time. The aim of the project is it to widen the existing 2- or 3-dimensional analysis methods (2D gel electrophoresis coupled with MALDI-TOF mass spectrometry by adding a new criterion of identification based on Surface Enhanced Raman Spectroscopy as well as to adapt a new method of fragment-free sample injection for the species-selective, mass spectrometric detection. For this purpose, existing expert knowledge of the project partners in the fields of quantitative Raman spectroscopy (Laser-Laboratorium Göttingen e. V.), multivariate chemo metric spectral analysis processes (LLG-GmbH) and laser inducted liquid beam desorption mass spectrometry (Microliquids GmbH) is to be bounded to develop a novel multi dimensional analysis system with a highly increased selectivity, sensitivity and a high automating potential. Next to the application areas in proteome research (particularly the field of pharmaceutical development) the planned system has a great potential of application in clinical diagnostics because of its multidimensional analysis structure. Mainly to mention is here the disease pattern recognition (DPR) which became more and more important during the last years.
	Enrollee: Laser-Laboratorium Göttingen GmbH
	Prof. Dr. Gerd Marowsky and Dr. Uwe Wachsmuth
	Hans-Adolf-Krebs-Weg 1
	D-37077 Göttingen
	Tel.: +49-551-5035-30
	Fax: +49-551-5035-99
	jutta.steckel at llg-gmbh.de
	The LLG GmbH is a company founded by the Laser Laboratory Göttingen e. V. In January 2004, an annex of 2350 m2 to the old LLG e. V. building was inaugurated. The building was designed as a centre for photonics, technology and communication; some highly innovative photonic companies do rent rooms in this building. Next to several services and the running of the new technology centre, the LLG GmbH plans to develop, produce and distribute own products in the fields of photonics. The biophotonics applied within this context are to be a new developmental sector in our company.
	Cooperation partner I: Microliquids GmbH
	Dr. Tim Spangenberg and Prof. Dr. Bernd Abel
	Hans-Adolf-Krebs-Weg 1
	D-37077 Göttingen
	Tel.: +49-551-30724-160
	Fax: +49-551-30724-162
	abel at microliquids.com
	Microliquids GmbH was founded in Summer 2003. Staff and associates of Microliquids GmbH have been collecting experiences in the fields of generation and manipulation of micro liquid beams and drops in the vacuum for 15 years. Micro liquid beams made out of different media (water, alcohols, liquid gases etc.) can be used in mass spectroscopic bioanalytics, in the generation of extreme ultraviolet radiation (EUV) and in photoelectron spectroscopy. The company develops and sells standard systems as well as client-specific system solutions for technologies in the fields of liquid micro beams and droplets in the vacuum.
	Cooperation partner II: Laser-Laboratorium Göttingen e.V.
	Dr. Volker Beushausen
	Hans-Adolf-Krebs-Weg 1
	D-37077 Göttingen
	Tel.: +49-551-5035-23

	volker.beushausen @ llg-ev.de
	Within the past 15 years, the Laser-Laboartorium Göttingen e. V. made a name for itself with successful work in the field of laser spectroscopic analysis system development for industrial fluidics utilization. The LLG e. V. was able to built on a huge know-how concerning imaging and sensory laser measurement techniques to analyze reactive and non-reactive flows in working for many years in BMBF associations led by the industry, application-oriented support programs of the federal state Lower Saxony, industrial research assignments and basic oriented DFG (German Research Foundation) projects. Particularly in the fields of laserinducted fluorescense and spontaneous Raman scattering, a huge resort of expertise exists.